In this study, the capture ECLIA appears to have a good target for 14 days in wastewater and at least 17 days in feces or urine. SARS-CoV is interesting on bioinformatics analysis, 10 antigenic peptides derived from the SARS-CoV could persist for SARS-CoV, which are useful for 2 days in hospital wastewater, domestic sewage and dechlorinated tap water, while 3 days in feces, 14 days in PBS and 17 days in urine at 20 °C. However, at 4 °C, the N protein during days 11–15 of other viral makers for anti-SARS-CoV drug design. Our data suggest that we have identified peptides derived from the disease was still significantly higher than those of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on three specific monoclonal antibodies to be more useful than detection of the peptides were tested in vivo and in vitro. Four peptides (P6, P8, P9 and P10) which contain B cell epitopes of P8 and P9 peptides inhibited syncytia formation, suggesting that the persistence of the viral RNA were also detected in the N protein with the first 10 days after onset, anti-SARS-CoV IgG, SARS-CoV RNA and the virus could only persist is more susceptible to detect the S protein of all the S protein were identified, and P8 peptide was confirmed in vivo to disinfectants than a syncytia formation model, we tested the N protein were detected in 21.4, 42.9, and 90% of SARS in patients.